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KMID : 0361920060360060422
Korean Journal of Orthodontics
2006 Volume.36 No. 6 p.422 ~ p.433
In vitro cytotoxicity of self-etching primers
Rhee Chang-Hoon

Kim In-Ryoung
Kim Gyoo-Cheon
Kim Seong-Sik
Son Woo-Sung
Abstract
Several ions and components are released from self-etching primers in the oral cavity. This may cause injury to the periodontal tissues throughout orthodontic treatment. The purpose of this study was to assess the cytotoxicity of self-etching primers to HGF-1, HaCaT, and RHEK cells.

Methods:Transbond XT Primer (3M Unitek, Monrovia, CA, USA), and self-etching primers, Clearfil SE Bond (Kuraray, Osaka, Japan), Transbond Plus SEP (3M Unitek, Monrovia, CA, USA), and Adper Prompt L-Pop (3M Unitek, Monrovia, CA, USA), were evaluated by MTT assay, and cellular changes were also observed.

Results:In all cells after 72 hours with all primers, severe morphological changes such as atrophy and necrosis were observed. In the MTT assay using HGF-1, Clearfil SE Bond, Transbond XT Primer, Transbond Plus SEP, and Adper Prompt L-Pop were lined up in order of ascending cytotoxicity When using HaCaT, Clearfil SE Bond, Adper Prompt L-Pop, Transbond Plus SEP, and Transbond XT Primer were lined up in order of ascending cytotoxicity. When using RHEK, Clearfil SE Bond, Transbond XT Primer, Adper Prompt L-Pop, and Transbond Plus SEP were lined up in order of ascending cytotoxicity.

Conclusion:The result of this study shows that care is needed because self-etching primers show cytotoxic properties similar to conventional primers.
KEYWORD
Cytotoxicity, Self-etching primer
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